AHSAN HUSSAIN

House # 177, Street # 6, Sector F-8/1, Phase 8 Bahria Town, Rawalpindi

Cell no. +92-334-5357201, e-mail: [email protected]

PERSONAL INFORMATION

Father’s Name: Muhammad Fayyaz
Date of Birth: 12th Dec 1981, Gujranwala
Permanent Address: Street Jevan Mal Padri Mohallah Bokhtaywala, Gujranwala
N.I.C #: 34101-2323655-9
Domicile: Punjab
Religion: Islam
Nationality: Pakistani

OBJECTIVES

To seek a challenging position in a well-reputed organization/institution, where I can utilize my knowledge in the most constructive manner; prove to be productive as well as enhance my capabilities and understanding in various areas of Biological Sciences.

EDUCATION

• M. Phil. (Molecular Biology / Biochemistry)
Quaid-i-Azam University, Islamabad (2006).
1st sem Tissue Culture, Medical Microbiology,
Advances in Cell Biology
2nd sem Virology, Plant Taxonomy, Cell Organelle,

Research Project: Biological activities of Fagonia cretica L.

• M.Sc [Biology (Biochemistry / Molecular Biology)]
Quaid-i-Azam University, Islamabad (2003)
1st sem. Cell Biology, Biostats, General Genetics, General Biochemistry
2nd sem. Molecular Biology of Cell, Metabolic Biochemistry, Evolution, Research Techniques
3rd sem. Gene Manipulation, Protein Chemistry, Enzymology, Radiobiology
4th sem. Nucleic Acid Chemistry, Microbial Physiology & Biochemistry, Clinical Biochemistry, Research Project

Research Project: Purification and characterization of alkaline proteases from Aspergillue terreus

• B.Sc (Botany, Zoology, Chemistry)
Punjab University Lahore (Gujranwala Campus) (2001)

MOLECULAR PATHOLOGY LAB EXPERIENCE

• Diagnosis and mutation profiling of Cancer patients for CALR, JAK2, MPL, Hairy Cell Leukemia, NRAS & KRAS Genes respectively by Sanger Sequencing and fragment analysis in Pakistani Population.
• Diagnosis and mutation profiling of G6PD gene, vWF, beta thalassaemia by Sanger Sequencing in Pakistani Population.
• Expression analysis of BCR/ABL Gene Rearrangement, and comprehensive approach to Real Time PCR (ABI 7500).

FORENSIC CASEWORK EXPERIENCE

• Forensic Case work and Human STR Profiling for Pathan, Punjabi & Northern Area (Research Projects) with comprehensive approach to Genetic Analyzer (ABI 3130, 310 and Bioanalyzer 2100)
• Casework – Disaster Victim Investigation (2012-13 & 2015)

ISO DOCUMENTATION

• Documentation for ISO-15189 (Molecular Path Lab)
• ISO-17025 (Forensic Lab – In process)
• Calibration of Instrumentation (Volume, Temperature and Speed)

RESEARCH PUBLICATIONS

• Ayesha Khurshid, Saleem Ahmed Khan, Chaudhry Altaf Hussain, Ahsan Hussain, Hamid Saeed Malik and Parvez Ahmed. BRAF V600E Mutation in Hairy Cell Leukaemia A Single Center Descriptive Cross Sectional Study. Hematol Transfus Int J 2017, 5(4): 00124
• Ahsan Hussain, Suhaib Ahmed, Nadir Ali, Hamid S. Mailk, Mariam Anees, Altaf H. Chuahdry & Parvez Ahmed. Rare β-Globin Gene Mutations in Pakistan, http://dx.doi.org/10.1080/03630269.2017.1339612
• Ahsan Hussain, Abdul Mannan, Hassan Zubair and Bushra Mirza. Purification and characterization of alkaline protease from Aspergillus terreus. J.Chem.Soc.Pak., Vol. 32, No. 4, (2010), 497-504.
• Ahsan Hussain, Muhammad Zia, Bushra Mirza. Cytotoxic and Antitumor Potential of Fagonia cretica L. Turk. J. Biol., 31, (2007), 19-24.

WORKSHOPS AND CONFERENCES

• Hands on Training on “TruSight Myeloid (54 gene) Library preparation” 7th Nov 2019 in AFIP – Pakistan.
• Hands on Training on “Illumina Ampliseq BRCA Library preparation” from 5th Nov – 6th Nov 2019 in AFIP – Pakistan.
• Hands on Training on “Trusight Tumor-15 Library preparation” from 28th Apr – 02nd May 2019 in AGBL – Dubai.
• Hands on “Training and Introduction to NGS workflow” from 11 – 22nd Mar 2019 in ICCBS – HEJ Karachi.
• Organize Workshop on “Next Generation Sequencing Data Analysis” from 07-08 Aug 2018 in AFIP Rawalpindi.
• Participant in National Forensic Science Conference on 15-16 Feb 2018 in Islamabad.
• Participant of Workshop on “Next Generation Sequencing Data Analysis” from 01-03 Feb in COMSATS Islamabad.
• Rare beta thalassaemia mutations in Pakistan, paper was presented in 13th National Conference & Scientific Exhibition of PSH On 13th – 15th January 2011 at Karachi.
• Introduction to ISO 9001:2008 & ISO 15189:2007 Requirements & Implementation in clinical labs, 12th – 13th Feb 2010 in AFIP, Rawalpindi.
• Training on DNA Sequencing in Center of Excellence in Molecular Biology (CEMB) Lahore in July 2009.
• Participant of the “Sixth International Biennial Conference of Microbiology” 18-21st March 2007 in Quaid-i-Azam University Islamabad.
• Participant of the workshop on “Bioengineering and Fermenter Applications” on 15th Aug 2006 in Quaid-i-Azam University Islamabad.
• Participate & present poster in “5th International & 15th National conference of Chemistry” 24-27th Nov 2004 in Quaid-i-Azam University Islamabad.
• Participate & present poster in “4th International Science Conference” 6-9th Oct 2004 in Azad Kashmir University.
• Participant of the International Symposium on the “Medicinal Plants; Linkages Beyond National Boundaries” 7-9th Sep 2004 in National Agriculture Research Center, Islamabad.
• Participant of the workshop on the topic “Identification Maintenance and Preservation of Microorganisms” 11-14th May in Quaid-i-Azam University Islamabad.
• Trainee of “Management Course” launched by NPO, under the supervision of Ministry of Industry 11th Nov 2003 in Lahore.

MANAGEMT SKILLS

• Purchase of consumable and electromedical equipment for the Molecular and Forensic DNA lab from 2012 – till date.

PROFESSIONAL EXPERIENCE

• Currently working as “Scientific Officer” in Armed Forces Institute of Pathology (AFIP) Rawalpindi from 11th Aug 2007 – till date.

INTERESTS AND HOBBIES

• Traveling, reading literature and writing and photography.

REFERENCES

• Maj Gen (R) Dr Suhaib Ahmed
Department of Pathology, Riphah International, University, Islamabad, Pakistan
[email protected]

• Brig (R) Dr Nadir Ali
Department of Pathology, Kalsoom International Hospital, Islamabad, Pakistan
[email protected]

• Col Dr Nayyar Chaudhry
Department of Forensic DNA, Forensic Medical Science Laboratory, Armed Forces Institute of Pathology, Rawalpindi, Pakistan.
[email protected]