CYNTHIA DELA RUEDA GULAY
2309 Yakal St. Tuntungin, Los Baños, Laguna
Mobile no. +639757985637
Email Address: [email protected]

Objectives

To be an asset to the team and/or company by using and continually honing the knowledge and skills earned from trainings and works.

● Specializations and Skills
➢ Preparation of reagents/media for the analysis.
➢ Inventory and maintenance of supplies and equipments (spectrophotometer, gel electrophoresis apparatus, centrifuge, vortex, rocker plates, autoclave, sterilized water dispenser, gel-documentation system.
➢ Preparation of samples.
● Advance Laboratory Skills:
➢ DNA/RNA sampling, extraction and running.
➢ Polymerase Chain Reaction (PCR)
➢ Surveyor Mutation Assay
➢ Proficient in Protein Analysis, gel electrophoresis and western blotting.
● Data Management. Experienced in the documentation of experimental data (excel file and laboratory note book, Microsoft Office such as Word, Excel, Power Point, Familiar in using Tablets for data collection. Operating System like Windows 98, XP, 2000, 2003 2007, 2010, VISTA, Linux).
● Team Work. Experienced in working in large multi-cultural team meeting strict deadlines.
● Other Skills. Experienced in plant husbandry (seeding, transplanting and maintenance) and the physiological screening of mutant plants via vein density using MEADE microscope. Familiar with several types of photo editor.
● Familiar with different Laboratory works such as chemical preparation and different types processing of leaf samples.
● Familiar with different equipment’s tools and apparatus in laboratory.
● Familiar in different process from seed germination to harvesting, crop establishment, land preparation, seed processing from threshing counting and sorting.
● Familiar in different machines and uses of it, both in field, and laboratory and screen houses.
● Familiar in and different plants and seed processing.

Work Experiences:

• IPB (Institute of Plant Breeding), University of the Philippines Los Baños
Laboratory Aide II March 1, 2018 – January 14, 2019

➢ Assisting in laboratory works. DNA extraction, Polymerase Chain Reaction (PCR), Gel Electrophoresis and Gel extraction.
➢ Set-up laboratory, greenhouse and field experiments.
➢ Prepare stock solution, laboratory reagents and other experimental/project materials.
➢ Assist in the collection of data.
➢ Prepare and process administrative documents.
➢ Perform regular inventory of project needed supplies and other services.
➢ Perform other technical support services as required for effective and successful implementation of the project.

• IRRI (International Rice Research Institute), Sustainable Impact
Administrative Staff (Interim Appointee) January 15, 2018 – February 28, 2018
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➢ Encode information correctly (Microsoft Excel, Microsoft Word, MS Access)
➢ Revised the encoded information
➢ Ensured documents are properly registered
➢ Transcribe data
➢ Do another task assigned by requestor

• IRRI (International Rice Research Institute), Grain Quality Nutrition Center
Professional Service II (Gawa at Kalinga) February 13, 2017 – July 31, 2017

Sampling in BG and CL4, Assisting in laboratory works. DNA extraction, Polymerase
Chain Reaction (PCR), Surveyor Mutation Assay, Buffer/ Reagent Preparation, Gel Agarose Electro-
phoresis, Gel Extraction, Stomatal and Epidermal Counting, and also making Hydroponics.
Dehulling and Milling.

• IRRI (International Rice Research Institute), CESD.
Professional Service I (Gawa at Kalinga) June 22, 2016 – February 10, 2017

Data Encoder using MS access and Excel

• Urata Nouen L.T.D, 2406 Shimakofuji, Itoshima Shi, Fukuoka-Pref. JAPAN.
Agricultural Farmer February 2013 – February 2016

Monitoring the growth of mizuna (vegetable) to be harvest checking the weight and the quality of
The product to be packed and ship for export through Malaysia, Thailand and Hongkong, conduct
inventory of the products.

• IRRI (International Rice Research Institute), Transgenic Physiology Team/C4 Rice Center
Research Technician II January 24, 2012 – July 29, 2012

Worked as part of a multi-cultural laboratory assisting with the screening of transgenic rice lines
for the expression of C4 genes. Protein Analysis, Western Blotting, DNA extraction, Polymerase
Chain Reaction (PCR), Surveyor Mutation Assay, Buffer/ Reagent Preparation and Gel Agarose
Electrophoresis.

From leaf sampling to grinding of samples, harvesting and threshing of samples
in screen houses and seed counting.

Field and screen houses Leaf Sampling Block F and CS05 from seed germination, plant or crop scoring, leaf or plant sampling and harvesting.

Stomatal Related measurements, Stomatal density and Stomatal Length (width and height) to data gathering of all parameters.

Leaf width and SPAD measurements.

• IRRI (International Rice Research Institute), Transgenic Physiology Team, C4 Rice Center,
Professional Service Staff November 2010 – January 2012

Worked as part of a multi-cultural laboratory assisting with the screening
of transgenic rice lines for the expression of C4 genes.

From seed germination transplanting to crop establishment leaf sampling to
grinding of samples, harvesting and threshing of samples in screen houses
and seed counting.

Field and screen houses Leaf Sampling Block F and CS05 from seed germination,
plant or crop scoring, leaf or plant sampling and harvesting.

Vein Density using MAEDE Hand Held Microscope and Image J Software photo and
image editor.

Stomatal Related measurements, Stomatal density Length (width and height) to data gathering of all parameters.

Leaf width and SPAD measurements.

• IRRI (International Rice Research Institute) Crop and Environmental Sciences Division, Mutant Production Team, C4 Rice Project
Los Baños, Laguna
Emergency Research Technician I
March 2010 – November2010

Worked as part of a large team in the production and screening of mutant sorghum lines for C4-like traits. From seed germination to plant scoring leaf sampling to harvesting to threshing of samples.

• IRRI (International Rice Research Institute) CESD and PBGB
Contract Worker (TOPGEM) April 2009 – March 2010

From seed preparation seeds germination, soaking, sowing assists in field layout, transplanting, replanting seeds pulling, harvesting threshing, seed counting separation. Purification panicle counting to threshing Filled and Unfilled counting. Plant selection materials for cross pollination. Cross pollination of several varieties sanctioning of plants that to be cross with other varieties, harvesting and threshing of cross pollinated plants.

• TOSHIBA INFORMATION EQUIPMENT (PHILS.) INC.,
103 East Main Ave. Ext., Special Export Processing Zone, Laguna Technopark, Biñan,
Laguna 4024
Production Operator June 2008 – November 2008

Seminars and Workshops Attended:

• RICH TALK SERIES Resiliency in Crops and Human Health “Genome-guided characterization of Medium Chain Fatty Acid in coconut towards marker-assisted breeding” Institute of Crop Science College of Agriculture and Food Science-UPLB May 3, 2018

• RICH TALK SERIES Resiliency in Crops and Human Health
“Health Benefits from Coconut Medium-Chain Fatty Acid”
Institute of Crop Science College of Agriculture and Food Science-UPLB May 10, 2018

• Japanese Culture and Language
RHV Placement Agency Inc.,
Bacoor, Cavite
August 2012 to October 2012

• Google Docs Training
International Rice Research Institute
Los Baños, Laguna
February 06, 2012 to February 08, 201

• Agilent Cary 60 Spectrophotometer Training/Course
International Rice Research Institute
Los Baños, Laguna
February 6 – 8, 2012

• Uvitec Gel Documentation Training Course
International Rice Research Institute.
Los Baños, Laguna,
February 6 – 8, 2012

Academic Background:

• Los Baños National High School
Batong Malake, Los Baños, Laguna.
June 2001 – April 2005

• Paciano Rizal Elementary School
Tuntungin, Los Baños, Laguna.
June 1995 – April 2001

Personal Identification:
Date of Birth:
January 21, 1989
Age:
30
Place of Birth:
Tuntungin, Los Baños, Laguna
Sex:
Female
Civil Status:
Married
Religion:
Roman Catholic
Language:
Filipino. English, Japanese

References:

Anand Noel C. Manohar
Institute of Plant Breeding, UPLB
University Researcher
[email protected]
+639163425800
Elizabeth Alcachupas
International Rice Research Institute
Data Management Specialist
[email protected]
+639176034768

​
Shiela Ann Edith Franco
International Rice Research Institute
Researcher
[email protected]
+639164020130

I hereby certify that the above information is true and correct up to the best of my knowledge.

Cynthia dela Rueda Gulay
Applicant​