M: +971554965305, E-Mail: [email protected]
Gulf pharmaceuticals Ras Al khaimah UAE PO BOX NO 997
9 years of strong industrial experience in downstream process. (Production & process development of insulin and glargine molecules.
· Strong experience in chromatography system and TFF system of various make and scale.
· Prioritizing in experiment design and process optimization.
GULF PHARMACEUTICALS (JULPHAR), UAE
Designation: Senior production biotechnologist
Department: Production (Downstream process)
MARCH 2012 – Till date
Ø Process and development of recombinant protein molecule (Insulin).
Ø Validation of process and cleaning.
Ø Optimization of TFF process.
Ø Optimization of centrifuge process as per process requirements.
Ø Operation of lyophilizer.
Ø Involved in optimization of lyophilization process as per working molecule.
Ø SOP preparation.
Ø Online completion, review and submission of MFM.
Ø Involved in pilot scale experiments as per process development.
Ø Autoclave and DHS validation as per process requirements.
Ø Analyzing the problem and troubleshooting.
VIVO BIOTECH HYDERBAD
Designation: Research Assistant
Department: Downstream process and cell biology
FEB 2008 – JAN 2012
Ø Protein Purification:
Ø Cell Lysis methods: Enzymatic, Sonication.
Ø Inclusion Bodies isolation, purification and solubilisation.
Ø Concentration and purification of Proteins by TFF and Dialysis
Ø Protein refolding methods.
Ø Chromatography methods.
Ø Analytical method development:
Ø Electrophoresis: SDS-PAGE, Isoelectric focusing.
Ø Immunoblotting: Western blot.
Ø Quantitative protein estimation method
Ø UV/VIS Spectrophotometry.
Ø Shake flask culture
Ø Molecular Biology and cell biology:
Ø Preparation of different media for mammalian cell lines
Ø Animal Cell Culture techniques
Ø Primary and secondary cell culturing
Ø Isolation of DNA, RNA, Plasmid
Ø Viability assays like MTT Assay, XTT assay Cell counting using Haemocytometer
Ø Enzymatic assay
Ø Western Blotting
Ø SDS PAGE
Ø Handling of different mammalian cell lines
Ø Cryopreservation and revival of cryopreserved mammalian cells lines.
Ø In vitro Biological activity determination of recombinant proteins by mammalian cell line
Ø Buffer preparation
Ø Preparation of cell culture reagents, antibiotics
UNIVERSITY OF HYDERABAD
Designation: Junior Research Fellow
Department: PLANT MOLECULAR BIOLOGY
FEB 2005 – APRIL 2007
Ø Isolation of DNA, RNA, Plasmid.
Ø Gene transformation methods in plant and Bacteria.
Ø PCR, RAPD, RFLP.
EQUIPMENT AND INSTRUMENT HANDLING
Ø AKTA Pilot, Purifier, Bioprocessor. : GE
Ø Chromatography system and column : Pall and GE.
Ø TFF system : Pall and GE.
Ø Centrifuge : GEA
Ø Lyophilizer : Martin Christ.
Ø Integrity Test machine : Pall.
Ø Operation of HMI and DCS
Ø M.Sc., (Microbiology) in 2000 – 2002 with – 71% from Bangalore University.
Ø B.Sc., (Microbiology) in 1997 – 2000 with – 69% from Kakathiya University.
Ø Board of Intermediate (BiPC) in 1996 – 1997.
Name : Rajendar.N
Father Name : Keshavulu
Date of birth : 11.01.1976
Marital status : Married
Nationality : Indian
Permanent Address : Post nagaram, mandal Hasanparthy, Dt, Warangal, Telangana, India
Ø Mr. KANNAN,
Gulf pharmaceuticals (Juphar XI)
Mail Id: [email protected]
The information furnished above is true to best of my knowledge and belief.
anywhere biotechnology protein purification division
before having five years experiance at india