RAJENDAR.N

M: +971554965305, E-Mail: [email protected]

Gulf pharmaceuticals Ras Al khaimah UAE PO BOX NO 997
JOB PROFILE

9 years of strong industrial experience in downstream process. (Production & process development of insulin and glargine molecules.
·         Strong  experience in chromatography system and TFF system of various make and scale.

·         Prioritizing in experiment design and process optimization.

GULF PHARMACEUTICALS (JULPHAR), UAE

Designation: Senior production biotechnologist

Department: Production (Downstream process)

MARCH 2012 – Till date

CAREER PROGRESSION

Responsibilities:

Ø       Process and development of recombinant protein molecule (Insulin).

Ø       Validation of process and cleaning.

Ø       Optimization of TFF process.

Ø       Optimization of centrifuge process as per process requirements.

Ø       Operation of lyophilizer.

Ø       Involved in optimization of lyophilization process as per working molecule.

Ø       SOP preparation.

Ø       Online completion, review and submission of MFM.

Ø       Involved in pilot scale experiments as per process development.

Ø       Autoclave and DHS validation as per process requirements.

Ø       Analyzing the problem and troubleshooting.

VIVO BIOTECH HYDERBAD

Designation: Research Assistant

Department: Downstream process and cell biology

FEB 2008 – JAN 2012

 
Responsibilities:

Ø  Protein Purification:

Ø Cell Lysis methods: Enzymatic, Sonication.

Ø Inclusion Bodies isolation, purification and solubilisation.

Ø Concentration and purification of Proteins by TFF and Dialysis

Ø Protein refolding methods.

Ø Chromatography methods.

Ø  Analytical method development:

Ø  Electrophoresis: SDS-PAGE, Isoelectric focusing.

Ø  Immunoblotting: Western blot.

Ø  Quantitative protein estimation method

Ø  UV/VIS Spectrophotometry.

Ø  Microbiology:

Ø  Shake flask culture

Ø  Molecular Biology and cell biology:

Ø  Microscopy

Ø  Preparation of different media for mammalian cell lines

Ø  Animal Cell Culture techniques

Ø  Primary and secondary cell culturing

Ø  Isolation of DNA, RNA, Plasmid

Ø  Viability assays like MTT Assay, XTT assay Cell counting using Haemocytometer

Ø  Enzymatic assay

Ø  AGE

Ø  Western Blotting

Ø  SDS PAGE

Ø  ELISA

Ø  Handling of different mammalian cell lines

Ø  Cryopreservation and revival of cryopreserved mammalian cells lines.

Ø  In vitro Biological activity determination of recombinant proteins by mammalian cell line

Ø  Buffer preparation

Ø  Preparation of cell culture reagents, antibiotics

UNIVERSITY OF HYDERABAD

Designation: Junior Research Fellow

Department: PLANT MOLECULAR BIOLOGY

FEB 2005 – APRIL 2007

Ø  Responsibilities:

Ø   Isolation of DNA, RNA, Plasmid.

Ø  Gene transformation methods in plant and Bacteria.

Ø  PCR, RAPD, RFLP.

EQUIPMENT AND INSTRUMENT HANDLING

Ø  AKTA Pilot, Purifier, Bioprocessor.           :              GE

Ø  Chromatography system and column     :               Pall and GE.

Ø  TFF system                                                         :               Pall and GE.

Ø  Centrifuge                                                          :               GEA

Ø  Lyophilizer                                                          :               Martin Christ.

Ø  Integrity Test machine                                   :               Pall.

Ø  Operation of HMI and DCS

ACADEMIC QUALIFICATION

Ø  M.Sc., (Microbiology) in 2000 – 2002 with – 71% from Bangalore University.

Ø  B.Sc., (Microbiology) in 1997 – 2000 with – 69% from Kakathiya University.

Ø   Board of Intermediate (BiPC) in 1996 – 1997.

PERSONAL DETAILS

Name                                : Rajendar.N

Father Name                     : Keshavulu

Date of birth                      : 11.01.1976

Marital status                     : Married

Nationality                         : Indian

Permanent Address          : Post nagaram, mandal Hasanparthy, Dt, Warangal, Telangana, India

REFERENCE:

Ø    Mr. KANNAN,

Purification manager,

Gulf pharmaceuticals (Juphar XI)

RAK, UAE.

Mail Id: [email protected]

Ph.no:+971553236213

DECLARATION

The information furnished above is true to best of my knowledge and belief.

N.RAJENDAR

s